ISO 22196: General Information
The International Organization for Standardization (ISO) is a worldwide federation of national standards bodies that develops international standards for the production and assessment of a variety of materials and processes. The ISO method 22196 is a quantitative test designed to assess the performance of antimicrobial finishes on hard, non-porous surfaces.
The method can be conducted using contact times ranging from ten minutes up to 24 hours. For an ISO 22196 test, non-antimicrobial control surfaces are used as the baseline for calculations of microbial reduction. The method is versatile and can be used to determine the antimicrobial activity of a diverse array of surfaces including plastics, metals, and ceramics.
The test substances (Nycote 7-11) were received on June 17, 2020.
Test Microorganism Information
The test microorganism selected for this test:
1. Escherichia coli 8739
This bacteria is a Gram-negative, rod shaped, facultative anaerobe commonly found in the gastrointestinal tract of mammals. Although most serotypes of this microorganism are harmless, there are pathogenic groups of E. coli such as enterohemorrhagic (EHEC), verocytotoxin producing (VTEC), and Shiga-like toxin producing (STEC) that can cause a multitude of illnesses. E. coli is relatively susceptible to disinfection when dried on a surface, yet it can be a challenging microorganism to mitigate in solution.
2. Staphylococcus aureus 6538
This bacterium is a Gram-positive, spherical-shaped, facultative anaerobe. Staphylococcus species are known to demonstrate resistance to antibiotics such as methicillin. S. aureus pathogenicity can range from commensal skin colonization to more severe diseases, such as pneumonia and toxic shock syndrome (TSS). S. aureus is commonly used in several test methods as a model for gram-positive bacteria. It can be difficult to disinfect, but does demonstrate susceptibility to low-level disinfectants.
Criteria for Scientific Defensibility of an ISO 22196 Study
For the laboratory to consider an ISO 22196 study to be scientifically defensible, the following criteria must be met:
- The average number of viable bacteria recovered from the time zero samples must be approximately 1 x 104 cells/cm2 or greater.
- Ordinary consistency between replicates must be observed for the time zero samples.
- The number of viable bacteria recovered from the control surface after the contact time must not be significantly (>2-Log10) less than the original inoculum concentration.
- Positive and Growth controls must demonstrate growth of appropriate test microorganism.
- Negative and Purity controls must demonstrate no growth of test microorganism.
Testing and results completed by an independent U.S. laboratory which maintains EPA compliance, FDA Good Laboratory Practices (GLPs) and ISO 17025 accreditation through ANSI National Accreditation Board (ANAB).
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